Morgan Gibbs-White, Ezri Konsa, and Kobbie Mainoo have been ruled out of England’s Nations League fixtures in October.
However, team captain Harry Kane has been declared fit by England’s medical team after his early substitution during Bayern Munich’s draw with Eintracht Frankfurt on Saturday.
The Three Lions are set to face Greece at Wembley on Thursday, followed by a match against Finland in Helsinki on Sunday.
The remaining 22 players in Lee Carsley’s initial squad have all arrived at St George’s Park, and interim manager Carsley has no plans to bring in any replacements.
Nottingham Forest midfielder Gibbs-White, who made his England debut last month, sustained an injury while taking a shot that was blocked during their 1-1 draw with Chelsea.
Aston Villa defender Konsa picked up a hamstring injury in Sunday’s goalless match against Manchester United, while United’s midfielder Mainoo was substituted late in the same game.