Scotland striker Lyndon Dykes will miss Euro 2024 due to a training ground injury sustained on Friday.
The 28-year-old has scored nine goals in 36 appearances and featured in all eight Euro qualifiers, starting five and scoring once.
Scotland’s tournament begins against hosts Germany on June 14.
Che Adams and Lawrence Shankland are the only other center-forwards in Steve Clarke’s squad.
“All the boys are gutted for Lyndon, it’s just a freak thing to happen,” midfielder Ryan Christie told BBC Scotland a few hours prior to confirmation of Dykes’ enforced withdrawal.
“We’ve had a few injuries already, so it’s frustrating.
“He’s been off for a while with the Championship [finishing on 4 May] and I know how hard he worked on his weeks off to prepare for this tournament.
“It’s just one of those things. There’s not much we can say to him at this point. We’re just trying to get behind him and I’m sure he’ll be supporting on.
“It adds another reason for us to be desperate to do well for him and the rest of the country.”
Scotland are already without Brentford’s Aaron Hickey, Everton’s Nathan Patterson, and Bologna’s Lewis Ferguson due to injuries.
Assistant manager John Carver mentioned on Thursday that all players who joined the provisional 28-man squad were “in good condition,” but he cautioned them against taking risks in training, as Scotland “can’t afford” more injuries.
Southampton midfielder Stuart Armstrong and Rangers center-half John Souttar trained separately on Thursday after missing the end of the season with their clubs.
Manchester United midfielder Scott McTominay, Nottingham Forest center-back Scott McKenna, Celtic left-back Greg Taylor, and Liverpool winger Ben Doak were absent from the first day of training.
Uncapped Doak has only recently resumed training following knee surgery in December, while midfielder Ryan Jack has played just 13 minutes for Rangers since February 10.