Barcelona’s 17-year-old defender Pau Cubarsi has been excluded from Spain’s final squad for Euro 2024.
Midfielders Marcos Llorente of Atletico Madrid and Aleix Garcia of Girona also miss out after Luis de la Fuente reduced his provisional squad to 26.
Former Newcastle and Leicester City forward Ayoze Perez, who scored on his international debut on Wednesday, will travel to Germany.
Premier League players included are Manchester City midfielder Rodri, Chelsea defender Marc Cucurella, and Arsenal goalkeeper David Raya.
Spain will play Northern Ireland in a final friendly match on Saturday before starting their Euro 2024 campaign on 15 June against Croatia.
They will also face Italy and Albania in Group B.
Full squad:
Goalkeepers: Unai Simon (Athletic Bilbao), Alex Remiro (Real Sociedad), David Raya (Arsenal – on loan from Brentford)
Defenders: Dani Carvajal (Real Madrid), Jesus Navas (Sevilla), Aymeric Laporte (Al-Nassr), Nacho Fernandez (Real Madrid), Robin Le Normand (Real Sociedad), Dani Vivian (Athletic Bilbao), Alex Grimaldo (Bayer Leverkusen), Marc Cucurella (Chelsea)
Midfielders: Rodri (Manchester City), Martin Zubimendi (Real Sociedad), Fabian Ruiz (Paris St-Germain), Mikel Merino (Real Sociedad), Pedri (Barcelona), Alex Baena (Villarreal), Fermin Lopez (Barcelona)
Forwards: Alvaro Morata (Atletico Madrid), Joselu (Real Madrid), Dani Olmo (RB Leipzig), Nico Williams (Athletic Bilbao), Mikel Oyarzabal (Real Sociedad), Ayoze Perez (Real Betis), Ferran Torres (Barcelona), Lamine Yamal (Barcelona)